The Mouse IgG ELISA Kit is an in vitro immunoassay for the quantification of IgG circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells. Specificity: The antibodies used in this kit have been shown by immunoelectrophoresis and ELISA to react specifically with IgG, and have essentially no reactivity with IgM, IgA, IgE or any other mouse serum proteins. Precision: IgG concentrations were measured with very good within-assay (4.4 to 7.3 %CV) and good between-assay (9.7 to 11.0 %CV) reproducibility. Kit Components: I1904-15A Microtiter Plate 1x96 wells I1904-15B Sample Diluent Concentrate 20X 1x10ml I1904-15C Wash Solution Concentrate 100X 1x10 ml I1904-15D IgG (HRP) 100X 1x150ul I1904-15E Standard 10ng/ml 1x650ul I1904-15F Standard 25ng/ml 1x650ul I1904-15G Standard 50ng/ml 1x650ul I1904-15H Standard 100ng/ml 1x650ul I1904-15J Standard 200ng/ml 1x650ul I1904-15K Positive Control 1x650ul I1904-15L TMB Substrate 1x12ml I1904-15M Stop Solution 1x12 ml Storage and Stability: May be stored at 4C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
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