Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:500-1:8000 Western Blot: 1:500-1:8000 Immunohistochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Reinheit:
Purified by immunoaffinity chromatography. ~90% by SDS-PAGE. May contain small amounts of intact IgG.
Formulierung:
Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zinc chloride, 1% BSA (Protease/IgG free), 0.05% sdium azide. Labeled with Alkaline Phosphatase (AP).
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