Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:200-1:5,000 Western Blot: 1:200-1:5,000 Immunohistochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by immunoaffinity chromatography. ~90% by SDS-PAGE. May contain small amounts of intact IgG.
Formulierung:
Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease/IgG free), 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP). Reconstitute with sterile ddH2O.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
