Application(s): Suitable for FLISA, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilution(s): Not specified. Optimal titers for applications should be determined by the researcher. Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Donkey IgG Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Prepared from monospecific antiserum by immunoaffinity chromatography using sheep IgG coupled to agarose beads.
Formulierung:
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 25% glycerol. FITC conjugated IgG.
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