Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG Fab molecule Applications: Suitable fo ruse Western Blot, ELISA, immunohistochemistry, immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:64,000 to 1:256,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by Immunoaffinity chromatography.
Formulierung:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerisol. Labeled with Biotin. Reconstitute with 1ml sterile, distilled, deionized water.
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