Applications: Suitable for use in Immunoblot (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:20,000-1:80,000 Western Blot: 1:20,000-1:80,000 Immunohistochemistry: 1:20,000-1:80,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by Immunoaffinity chromatography.
Formulierung:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% gentamicin sulfate. Reconstitute in 500ul sterile ddH2O. Labeled with horseradish peroxidase (HRP).
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