Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1500-1:10,000. Assayed against 1ug of mouse IgG in a standard ELISA using NPP as a substrate for 30 minutes at RT. Western Blot: 1:500- 1:2500 Immunohistochemistry: 1:200- 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase
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