Applications: Suitable for use in Immunoblot (Western and Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000-1:5000 ELISA: 1:50,000 Immunohistochemistry: 1:500-1-2500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (Immunoglobulin and Protease free), 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
