Applications: Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Other applications not tested. Recommended Dilution: ELISA: 1:30,000-1:150,000, I1904-83N was assayed against 1ug of mouse IgG2a in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS (2,2-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Optimal dilutions to be determined by the researcher. Reconstitution: Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
