Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:20-1:200 Western Blot : 1:20-1:200 ELISA: 1:200-1:1000 Immunohistochemistry: Formalin fixed paraffin embedded. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified IgG fraction to a purity of > 95%.
Formulierung:
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP)
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