IL1 is a major regulator in both innate and adaptive immune responses, plays an important role in the regulation of acute inflammation and has also been implicated in many other processes, including both fever and fibrinogen induction. The previous names by which IL1 has been known includes lymphocyte activating factor, endogenous pyrogen, catabolin, hemopoietin1, melanoma growth inhibition factor, and osteoclast activating factor, reflecting its widespread cell expression. Studies using chicken models have indicated physiological similarities between chIL1beta and mammalian IL1beta in relation to its neural distribution and several factors have been shown to induce chIL1beta secretion, including protein kinase C (PKC), Calmodulindependent protein kinase, lipopolysaccharide, phorbol esters, calcium and silica. Applications: Suitable for use in ELISA, Functional Assays and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: Detects a band at ~29kD in HD-11 cell lysates transfected with chIL-1. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
