Recombinant protein corresponding to aa117-269 from human IL-1b expressed in E. coli.
Interleukin-1B (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Human IL-1beta is a 17kD protein containing 153 amino acid residues. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization of Bioactivity. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml using recombinant human IL-1b Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood lymphocytes and immersion fixed human peripheral blood mononuclear cells treated with LPS and monensin. Neutralization: Measured by its ability to neutralize IL-1 beta /IL-1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 6-42 ng/mL in the presence of 50 pg/mL Recombinant Human IL-1 beta /IL-1F2 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
