Interleukin 1 Receptor Type I (IL-1RI) (Carboxyfluorescein), IgG2b, Clone: [7H42], Rat, Monoclonal
Artikelnummer:
USB-I7663-25G2
Hersteller Artikelnummer:
I7663-25G2
Alternativnummer:
USB-I7663-25G2-100
Hersteller:
US Biological
Wirt:
Rat
Kategorie:
Antikörper
Applikation:
FC
Immunogen:
Recombinant mouse IL-1 RI extracellular domain.
Two distinct types of receptors that bind the pleiotropic cytokines IL-1a and IL-1b have been described. The IL-1 receptor Type I is an 80kD transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68kD transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1a or IL-1b, but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. 10ul labels 10e5 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Carboxyfluorescein conjugates are sensitiv
