Human endothelial interleukin-8 (IL-8) is an 8.9kD protein containing 77 amino acid residues. It promotes neutrophil chemotaxis and degranulation. The 77 amino acid IL-8 is the predominant form secreted by endothelial cells. Recombinant protein corresponding to human IL-8, expressed in E. coli Biological Activity: Determined by its ability ot chemoattract human peripheral blood neutrophils using a concentration of 25-150ng/ml. Amino Acid Sequence: AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Country of Origin: USA
Molekulargewicht:
8.9
Reinheit:
98% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug
Formulierung:
Supplied as a lyophilized powder without additives. Reconstitute with sterile dH2O to a concentration of 0.1-1.0mg/ml. Do not vortex.
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