| IL-9 is an immunoregulatory cytokine produced by IL-2 activated Th2 lymphocytes. IL-9 enhances the proliferation of T lymphocytes, mast cells, erthroid precursor cells and megakaryoblastic leukemia cell lines. Over-expression of IL-9 has been implicated in the pathogenesis of anaplastic lymphoma and Hodgkins disease. Whereas murine IL-9 can function on human cells, human IL-9 is inactive on mouse cells. Recombinant human IL-9 is a 14.kD protein of 127 amino acid residues including 10 cysteine residues which are fully conserved between the human murine proteins. Recombinant protein corresponding to human IL-9, expressed in E. coli. Amino Acid Sequence: MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI Biological Activity: Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is <0.2ng/ml, corresponding to a specific activity of >5x10e6units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
