Recombinant protein corresponding to Ile21-Ser220 from human IL-10R beta, expressed in NS0 cells Species Sequence Homology: rat, 87%, human, 74%
IL-10 R-beta (Interleukin 10 receptor beta, also IL-10R2, CD210b and CRF2-4) is an 80-85kD member of the type II cytokine receptor family of proteins. It is very widely expressed and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B, and -29 (plus IL-26 in human). Mature mouse IL-10 R-beta is a type I transmembrane protein with an extracellular domain that possesses two fibronectin type III domains (aa22-107 and 11-208). There is one alternate start site at Met20. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml/10e6 cells using EL-4 mouse lymphoblast cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
