Synthetic peptide corresponding to C-EARQQNKYENYS from the internal region of mouse Il12b/Il12p40 (NP_032378.1).
IL-12 is a heterodimer composed of subunits IL-12A p35 and IL-12B p40. Autocrine IL-12 is responsible for the differentiation of naive CD4+T cells into type 1 helper T cells (Th1) that produce interferon-_ (IFN-_). Expression of STAT 4, a signal transduction molecule important in cytokine-induced gene expression, appears central to IL-12 induced Th1 production of IFN-_. It also activates production of tumor necrosis factor (TNF) from T and natural killer cells. IL-12 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:4000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
