| Interleukin-15 (IL-15) is an immunomodulating cytokine that stimulated the proliferation of T lymphocytes. It shares many biological properties with IL-2. IL-5 is a potent lymphoid cell growth factor that exerts its biological activities primarily on T cells. It is essential in the development, survival and activation of NK cells. Increased expression of IL-15 has been implicated with rheumatoid arthritis, imflammatory bowl disease and diseases affiliated with retroviruses HIV and HTLV-I. Human IL-15 is a 12.9 kD protein containing 114 amino acid residues. Biological Activity: Fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of CTLL-2 cells was found to be 0.5ng/ml, corresponding to a specific activity of 2x10e6 units/mg. Amino Acid Sequence: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS Responding Cells (partial list): T cells, NK cells, LAK cells Recommended Dilutions: Most in vitro applications, IL-15 exerts its biological activity in the concentration range of 0.1-10ng/ml. Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
