Interleukin 5, Recombinant, Rat (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Produktübersicht

• Artikelname: Interleukin 5, Recombinant, Rat (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)
• Artikelnummer: USB-I8437-06
• Hersteller Artikelnummer: I8437-06
• Alternativnummer: USB-I8437-06-2,USB-I8437-06-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. Recombinant rat IL-5 is a 26kD disulfide-linked homodimeric protein containing two 113 amino acid chains. Recombinant protein corresponding to rat IL-15, expressed in E. coli. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.5ng/ml, corresponding to a specific activity of 2x10e6 units/mg. Amino Acid Sequence: MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: 98% by RP-HPLC, SDS-PAGE. Endotoxin: 0.1ng/ug (<1EU/ug)
• Formulierung: Supplied as a lyophilized powder from 5mM sodium phosphate, pH 7.5. Reconsitute with sterile ddH2O to 0.1-1mg/ml. Do not vortex.