Interleukin 32 isoform A belongs to cytokine family. This protein expression is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. It has the ability to induce proinflammatory cytokines such as TNF_ and IL8 in THP1 cells, and activates typical cytokine signaling pathways involving NF_B and p38. IL-32 can also support osteoclast differentiation but not osteoclast activation by regulating the MAPK/ERK pathway and the actin cytoskeleton. Recombinant protein corresponding to aa1-131 of human Interleukin 32, fused to His-tag at N-terminus, was expressed in E. coli. Molecular Weight: 19.1kD (168aa) Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMCF PKVLSDDMKK LKARMHQAIE RFYDKMQNAE SGRGQVMSSL AELEDDFKEG YLETVAAYYE EQHPELTPLL EKERDGLRCR GNRSPVPDVE DPATEEPGES FCDKSYGAPR GDKEELTPQK CSEPQSSK Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
