A recombinant protein derived from the C-terminal fragment of human IQGAP1 protein.
Proteins of the IQGAP family have been identified as candidate effectors for the Rho family of GTPases. The domain structure of IQGAP family members suggests that these proteins may function as regulators of the cytoskeleton. In particular their sequences include 1) an actin-binding domain homologous to that found in calponin, 2) IQ motifs for interaction with calmodulin, and 3) a GTPase-binding domain. Two IQGAP isoforms have been identified, IQGAP1 and IQGAP2. These proteins share 62% sequence identity. The 189-kD IQGAP1 protein exhibits a broad tissue distribution and is especially enriched in placenta lung and kidney. On the other hand, the 180-kD IQGAP2 protein is restricted in its tissue distribution and appears to be expressed exclusively in liver. Both IQGAP1 and IQGAP2 bind to GTP-bound Cdc42 and inhibit the rate of GTP hydrolysis. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.1-1ug/ml Western Blot: 1-2ug/ml Immunoprecipitation (native): 5ug/IP reaction Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3G170]
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
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