JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at Threonine and Tyrosine residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on S63 and S73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity. Source: Recombinant full-length mouse JNK1 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_016700. Molecular Mass: The approximate molecular weight is 71 kD (see Figure 1 below). Activity: The specific activity of JNK1 was determined to be 124 nmol/min/mg using an ATF2 substrate Storage: This product is stable at -70 C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
