KIR2DS4, Fc Chimera, Recombinant, Human (Killer Cell Immunoglobulin-Like Receptor 2DS4, CD158 Antigen-like Family Member I, MHC Class I NK Cell Receptor, Natural Killer-associated Transcript 8, NKAT-8, P58 Natural Killer Cell Rece
KIR2DS4, Fc Chimera, Recombinant, Human (Killer Cell Immunoglobulin-Like Receptor 2DS4, CD158 Antigen-like Family Member I, MHC Class I NK Cell Receptor, Natural Killer-associated Transcript 8, NKAT-8, P58 Natural Killer Cell Rece
KIR2DS4, Fc Chimera, Recombinant, Human (Killer Cell Immunoglobulin-Like Receptor 2DS4, CD158 Antigen-like Family Member I, MHC Class I NK Cell Receptor, Natural Killer-associated Transcript 8, NKAT-8, P58 Natural Killer Cell Rece
Artikelnummer:
USB-K1858-60A
Hersteller Artikelnummer:
K1858-60A
Alternativnummer:
USB-K1858-60A-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
KIR2DS4 (2DS4), previously called NKAT-8 and designated CD158i, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they differentiate normal from abnormal cells and regulate effector functions in the innate immune system. KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. KIR2DS4 cDNA encodes 304aa including a 21aa signal sequence, a 224aa ECD, a 20aa transmembrane sequence and a 39aa cytoplasmic domain. KIR receptors have no structural orthologs in nonprimates, although mouse 49aa proteins perform similar functions. Like other activating KIRs, KIR2DS4 has a short tail and a positively charged aa within the transmembrane domain that interacts with the ITAM-bearing signaling adaptor, DAP12. Expression of activating KIR is balanced with inhibitory KIR to impart specificity to the immune response. KIR2DS4 recognizes six HLA-C allotypes and two HLA-A allotypes (A*1101 and A*1102) but no HLA-B allotypes. It has also been suggested that KIR2DS4 recognizes a non-MHC ligand, based on specific binding to melanoma cells that lack MHC Class I. A common allele, KIR2DS4*003, is present in up to 89% of Caucasians and ~30% of Asians, it creates a prematurely terminated, soluble, inactive form. Lack of functional KIR2DS4 can potentially create unbalanced inhibition of killer cell function. Recombinant protein corresponding to Gln22-His245 from human KIR2DS4, Accession: P43632, was fused to the carboxy-terminal Fc region of human IgG1 (Pro100-Lys330) via a linker peptide (IEGRMD). The chimeric protein was expressed in mouse myeloma cell line, NSO Molecular Weight: ~51.2kD Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
