Kpn2I (BspMII)

Produktübersicht

• Artikelname: Kpn2I (BspMII)
• Artikelnummer: USB-K1895-10
• Hersteller Artikelnummer: K1895-10
• Alternativnummer: USB-K1895-10-500,USB-K1895-10-2500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-T C C G G A-3 3-A G G C C T-5 Concentration: 10u/ul Source: E.coli that carries the cloned kpn2IR gene from Klebsiella pneumoniae RFL2 store at -20C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Kpn2I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Kpn2I, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.3uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.1% of white colonies were detected. Methylation Effects: Dam: may overlap- no effect Dcm: never overlaps- no effect CpG: completely overlaps- blocked EcpKl/EcoBl: never overlaps- no effect Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55C. Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: BshTI, BsaWI, Cfr9I, Cfr10I, Eco88I, NgoMI, SgrAI Number of Recognition Sites in DNA: Lambda: 24 PhiX174: 0 pBR322: 1 pUC57: 0 pUC18/19: 0 pTZ19R/U: 0 M13mp18/19: 0 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55C in 50ul of assay buffer.