D-Lactate dehydrogenase, isolated and purified from Lactobacillus leichmannii.
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Applications: Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by ammonium sulphate precipitation and ion exchange chromatography.
Formulierung:
Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 1ml sterile ddH2O.
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