Principle of the Assay: This is an ELISA for the quantitative analysis of 15-epi-Lipoxin A4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and 15-epi-Lipoxin A4 in the sample for a limited number of binding sites. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of 15-epi-Lipoxin A4 in the sample or standard. For example, the absence of 15-epi-Lipoxin A4 in the sample will result in a bright blue color, whereas the presence of 15-epi-Lipoxin A4 will result in little or no color development. Kit Components: L2622-10A: Coated Microwell plate, 1x96 wells L2622-10B: LXA4-15e Standard (1ug/ml), 1x50ul *L2622-10C: LXA4-15e Conjugate, 2x1vial L2622-10D: Extraction Buffer (5X), 1x30ml L2622-10E: ELISA Buffer, 1x30ml L2622-10F: Wash Buffer (10X), 1x20ml L2622-10G: TMB Substrate, 1x20ml Storage and Stability: Store *L2622-10C powder at -20C. Store other components at 4C. Stable for at 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten