Specific Activity: ~55 units/mg protein Definition of Unit: One unit is the amount of enzyme that catalyzes the conversion of one nanomole of arachidonic acid to 12- hydroperoxy eicosoteraenoic acid (12-HPETE) over 5 minutes incubation at RT. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months after receipt.
Formulierung:
Suspended in 500ul of 20mM Tris, pH 7.2, 0.5mM EDTA, PMSF, DTT, 15% glycerol.
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