| Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called renal secretion. Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides, oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Most recently, a novel liver-specific OAT, LST-1, has been cloned from human liver. It has also been detected in mouse and rat liver. No significant expression of LST-1 was detected in other tissues. Human LST-1 (691 aa, 12 TM) shares 42% homology with human OATP, rat oatp1-3, and OATK-1, and 35% with PGT. Phylogenetic analyses places LST-1 between oatp and PGT. LST-1 has wide substrate specificity (taurocholate, conjugated steroids, PGE2, Thromboxane B2, leukotrienes, and T3). Therefore, LST-1 may be more important for clearance of bile acids and organic anions in liver. Synthetic peptide corresponding to 17aa sequence near the C-terminus of human LST-1 Recognizes human Liver Specific Transporter. No significant sequence homology is detected with other OATP1-3, OATP or PGT. Applications: Suitable for use in Antibody Blocking and ELISA. Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: Antibody Blocking: 5-10ug/ug per 1ug of of affinity purified antibody (L3176-06) or 1ul of serum (L3176-07). ELISA: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
