| D-Luciferin potassium salt is a useful substrate for the enzyme Firefly Luciferase. Upon oxidation by the enzyme luciferase, produces bioluminescence. D-Luciferin can be utilized to assay the expression of the luciferase gene linked to a promoter of interest. Alternatively, D-Luciferin and luciferase can be used to assess ATP availability in cellular or biochemical assays. Synonyms: (S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid potassium salt,, 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt, D-Luciferin, potassium CAS No: 115144-35-9 Molecular Formula: C11H7N2O3S2K Molecular Weight: 318.42 Purity: 99% (HPLC) Appearance: Light yellow powder Solubility (1% H2O): Slightly yellow to yellow, clear, complete Turbidity of Solution (1% H2O): 4 NTU Water Content (KF): 1% TLC: 50mg/5ml water, ethanol/ammonia 20:1, UV 254nm FT-IR Spectrum: Conforms to reference Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
