| Lysozyme (muramidase) hydrolyzes preferentially the beta-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. A somewhat more limited activity is exhibited towards chitin oligomers. Lysozyme is of widespread distribution in animals and plants. That which has been most extensively studied is from hen egg white (lysozyme C). Lysozyme is also found in mammalian secretions and tissues, saliva, tears, milk, cervical mucus, leukocytes, kidneys, etc. Lysozyme is inhibited by surface-active reagents such as dodecyl sulfate, alcohols and fatty acids. Imidazole and indole derivatives are inhibitors via formation of change-transfer complexes. Useful for the rapid isolation of RNA from gram negative bacteria. Can be used to prepare small amounts of plasmid DNA. Source: Chicken egg white Synonyms: EC=3.2.1.17, Muramidase, Mucopeptide N-acetylmuramoyl hydrolase CAS No: 12650-88-3 Molecular Weight: 14,388 Appearance: Supplied as a white powder. Activity: 20,000u/mg Solubility: Dissolves readily at 5mg/ml in 0.1M potassium phosphate, pH 6.24 to give a clear, colorless and complete solution. pH: 3.0-4.5 Ash: 2% Moisture: 8% Total Bacteria: 5000cfu/g Yeast and Mold: 100cfu/g E. coli: 3MPN/g Salmonella: Negative/25g S. aureus: Negative/25g Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 6 months after receipt at -20C. Country of Origin: China |
