Lysozyme, Human Neutrophil (Muramidase), CAS [[9001-63-2]]
Artikelnummer:
USB-L9200-14
Hersteller Artikelnummer:
L9200-14
Alternativnummer:
USB-L9200-14-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Lysozyme (muramidase) hydrolyzes preferentially the beta-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. A somewhat more limited activity is exhibited towards chitin oligomers. Lysozyme is of widespread distribution in animals and plants. That which has been most extensively studied is from hen egg white (lysozyme C). Lysozyme is also found in mammalian secretions and tissues, saliva, tears, milk, cervical mucus, leukocytes, kidneys, etc. Useful for the rapid isolation of RNA from gram negative bacteria. Can be used to prepare small amounts of plasmid DNA. Purity: 95% by SDS-PAGE Appearance: Lyophilized from 0.05M sodium acetate, pH 6.0, 0.1M sodium chloride. Reconstitute with ~500ul ddH20. Activity: 100,000 units/mg protein (E1%/280) Unit Definition: One unit will produce a delta A450 of 0.001/min at pH 6.24 and 25C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6ml reaction mixture (1cm light path). Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Nummer:
[9001-63-2]
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