MDC is a CC chemokine that is produced in B cells, macrophages, monocyte-derived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC chemoattracts monocytes, dendritic cells and NK cells, and exerts HIV-suppressive activity. The 67 amino acid form of MDC displays reduced chemoattractant activity, but retains HIV-suppressive activity. Recombinant mouse MDC is a 7.8kD protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines. Recombinant protein corresponding to 68aa from mouse Macrophage Derived Chemokine expressed in E. coli. Molecular Weight: ~7.8kD Biological Activity: Determined by its ability to chemoattract murine BW5147.3 cells using a concentration range of 1-30ng/ml. Amino Acid Sequence: GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to 0.1-1mg/ml, which can then be further diluted to other aqueous solutions.
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