P44/42 MAPK (Erk1 and Erk2), SAPK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, and the differentiation and control of cellular responses to cytokines and stress. p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* resulting in p38 activation. MAPK Family Antibody Sampler Kit contains 20ul of each primary antibody (p44/42 MAP Kinase, SAPK/JNK, p38 MAP Kinase) and 50ul of anti-rabbit IgG secondary antibody labeled with HRP. Kit Components: M2352-40A: p44/42 MAP Kinase Antibody 1x20ul M2352-40B: SAPK/JNK Antibody 1x20ul M2352-40C: p38 MAP Kinase Antibody 1x20ul M2352-40D: Rabbit ant-Human IgG, HRP, 1x50ul Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by Protein A and peptide affinity chromatography.
Formulierung:
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
