MAPKAP Kinase 2, Inactive, Recombinant, Human (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)

Produktübersicht

• Artikelname: MAPKAP Kinase 2, Inactive, Recombinant, Human (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)
• Artikelnummer: USB-M2355-07
• Hersteller Artikelnummer: M2355-07
• Alternativnummer: USB-M2355-07-50
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis. The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts. N-terminal, GST-tagged, fusion protein encoding aa36-371 of human MAPKAP Kinase 2 (MAPKAP-K2), expressed in E. coli Molecular Weight: ~66kD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 66
• NCBI: 004759
• UniProt: P49137
• Reinheit: 90% (SDS-PAGE)
• Formulierung: Supplied as a liquid in 50ml of 50mM Tris-HCl, pH 7.5, 0.15M sodium chloride, 0.1mM EGTA, 0.1% 2- mercaptoethanol, 0.02% Brij-35, 50% glycerol.