MARCKS, phosphorylated (Ser152/156) (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain, Phosphomyristin, PKCSL, PRKCSL, Protei
Artikelnummer:
USB-M2375-05E
Hersteller Artikelnummer:
M2375-05E
Alternativnummer:
USB-M2375-05E-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser152/156 of rat MARCKS, conjugated to KLH. Species Sequence Homology: bovine, chicken, human, xenopus and zebrafish
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser-152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994) Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 using rat brain lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
