| Microtubule associated proteins regulate the stability of microtubules, controlling processes such as cell polarity/differentiation, neurite outgrowth, cell division, and organelle trafficking (1). The MARK MAP/microtubule affinity-regulating kinases) family MARK1-4) of serine/threonine kinases were identified based on their ability to phosphorylate microtubule associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARKs phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimers disease. Over-expression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7). Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
