MELAN-A/MART-1 is a melanocyte differentiation marker. MELAN-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines. It is present in melanocytes of normal skin and retina, nevi and in more than 85% of melanomas. MELAN-A/MART-1 is not expressed by non-melanoma malignancies. It is recognized by autologous cytotoxic T lymphocytes. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Seven other melanoma-associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-1, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Applications: Suitable for use in Immunoprecipitation, Western Blotting, and Immunohistochemistry (frozen & formalin/paraffin). Other applications have not been tested. Recommended Dilution: Immunoprecipitation: Use Protein G, 2ug antibody/mg protein lysate Western Blotting: 1ug/ml for 2 hours at RT. Immunohistochemistry: 1-2ug/ml for 30 min. at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: CaC1 melanoma cells and melanomas. Cellular Localization: Cytoplasmic. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[0.N.399]
Isotyp:
IgG1
Reinheit:
Purified by Protein G affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
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