Recombinant protein corresponding to human MART-1.
MELAN-A/MART-1 is a melanocyte differentiation marker. MELAN-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines. It is present in melanocytes of normal skin and retina, nevi and in more than 85% of melanomas. MELAN-A/MART-1 is not expressed by non-melanoma malignancies. It is recognized by autologous cytotoxic T lymphocytes. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Seven other melanoma-associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-1, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Applications: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunoprecipitation: Use Protein A, 2ug antibody/mg protein lysate Western Blot: 0.5ug/ml Immunohistochemistry (frozen & formalin/paraffin): 2-4ug/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[0.N.396 + 0.N.397]
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide (Cat M2410-12).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
