Recombinant protein corresponding to Asp29-Arg486 from human Matrilin-3, expressed in NS0 cells.
Human Matrilin-3 is a secreted, 50-60kD member of the matrilin family of noncollagenous extracellular matrix proteins. It is 458 amino acids (aa) in length and consists of an N-terminal von Willebrand Factor-A domain (vWFA) (aa83-258), four EGF-like repeats (aa264-431), and a C-terminal 25aa a-helix/coiled-coil region. Matrilin-3 is cartilage specific and produced by chondrocytes. It forms both homotetramers and heterotetramers with matrilin-1. The vWFA domain in human matrilin-3 is 91%, 93%, 91%, and 93% aa identical to the vWFA domain in mouse, dog, rat and cow matrilin-3, respectively. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinnat human Matrilin-3 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
