Ovalbumin conjugated synthetic peptide corresponding to a region within the C-terminus of human MMP-1.
Matrix metalloproteinase 1 (MMP-1), also known as interstitial or fibroblast collagenase, is a 469 amino acid, in the pro-peptide form, zinc-dependent endopeptidase responsible for degrading the extracellular matrix. MMPs are also involved in cell proliferation, migration, differentiation and apoptosis (Chen et al. 2013). Most MMPs are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active (Nagase et al. 1992). MMP expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis (Murray et al. 1996). MMP-1 is one of the collagenases, capable of degrading collagens I, II and III, all main components of the interstitial stroma (Robichaud et al. 2011). MMP-1 is overexpressed in invasive melanoma, colorectal and esophageal cancers (Langenskiöld et al. 2013). MMP-1 has also been implicated in arthritis and may influence atherosclerotic lesion formation (Nikkari et al. 1995) . Additionally, MMP-1 has been implicated in the repair processes of the heart after myocardial infarction (Yarbrough et al. 2003). Has been used successfully for the detection of MMP-1 in gastric cancer by both Western blotting and immunohistochemistry. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot: detects a band of ~37kD Optimal dilutions to be determined by the researcher. Hybridoma: Ag8.653 myeloma cells with spleen cells from Balb/c mice. Positive Control: Colon carcinoma Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
