Matrix Metalloproteinase 7, Active, Recombinant, Human (MMP-7, Matrilysin, Pump1, Uterine Metalloproteinase)
Artikelnummer:
USB-M2423-20
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| Artikelname: | Matrix Metalloproteinase 7, Active, Recombinant, Human (MMP-7, Matrilysin, Pump1, Uterine Metalloproteinase) |
| Artikelnummer: | USB-M2423-20 |
| Hersteller Artikelnummer: | M2423-20 |
| Alternativnummer: | USB-M2423-20-10 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| H-MMP-7 is a member of the Matrix metalloproteinase family of endopeptidases, containing the canonical HExxHxxGxxH motif as a zinc binding active site. MMP-7 and MMP-26 (Matrilysin-2) are the only MMPs discovered that completely lack a hemopexin ''regulatory domain or hinge region. Like the other MMPs, MMP-7 is secreted as a zymogen, then activated. The 28kD form is reduced to 18kD by enzymatic cleavage after the conserved ''cysteine switch. MMP-7 is not constitutively produced. It is produced on demand in specific tissues. MMP-7 substrate specificity is broad, most closely resembling the activity of MMP-3 (Stromelysin-1). When MMP-7 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them. Applications: Suitable for use in ELISA, Western Blot (control or standard) and SDS-PAGE Standards. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Molecular Weight: 19,130 calculated from amino acid sequence and 20,000 from SDS-PAGE, followed by staining with CBB R-250. Specific Activity: ~5000 U/mg protein Unit Definition: One unit of enzyme activity is defined as the quantity required to digest 1ug of Azocoll/min. at pH 7.5 and 37C. Storage and Stability: MMP-7, when stripped of TIMPs, is unstable, and must stored carefully. To avoid repeated freeze thaw cycles, aliquot and store the enzyme at -70C. Aliquots are stable for 6 months after receipt at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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