Mbo I

Produktübersicht

• Artikelname: Mbo I
• Artikelnummer: USB-M2460
• Hersteller Artikelnummer: M2460
• Alternativnummer: USB-M2460-300,USB-M2460-1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5- G A T C-3 3-C T A G -5 Concentration: 10u/ul Source: Moraxella bovis Buffer: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA dam-x 16 hours) with MboI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with MboI, more than 90% of the DNA dam-, fragments can be ligated at a 5-termini concentration of 1.2uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Methylation Effects: MboI cuts neither Gm6ATC, GAThm5C nor GAhm5UC, but cuts GATm4C and GATm5C. Blocked by Dam methylation. Stability during Prolonged Incubation: A minimum of 0.1units of enzyme are required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Compatible Ends: BamHI, BclI, BglII, PsuI Number of Recognition Sites in DNA: Lambda: 116 PhiX174: 0 M13mp18/19: 7 pBR322: 22 pUC18/19: 15 pUC57: 15 pTZ19R/U: 15 pBluescriptIIKS(-/+): 15 pBluescriptIISK(-/+): 15 pACYC177: 22 pACYC184: 15 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 37C in 50ul of assay buffer.