MCP-2 (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8 Precursor, SCYA8, SCYA10) (Biotin), Rabbit
Artikelnummer:
USB-M2750-07
Hersteller Artikelnummer:
M2750-07
Alternativnummer:
USB-M2750-07-25,USB-M2750-07-50
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
Recombinant protein corresponding to human MCP-2, expressed in E. coli.
MCP-2 acts as a chemoattractant for monocytes, lymphocytes, eosinophils and basophils, due to its ability to bind to the common MCP receptor, chemokine (C-C motif) receptor 2 (CCR2), and also CCR1 and CCR3. Proteolytic cleavage of MCP-2 into the NH2-terminally truncated form, MCP-2 (6-76), results in the loss of chemotactic activity, forming a functional C-C chemokine inhibitor, which may be important during inflammatory responses. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Direct ELISA: 0.25-1ug/ml (using 100ul/well) using M2750-08A as capture antibody allows detection of at least 0.2-0.4ng/well of recombinant human MCP-2 Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilution to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
