Synthetic peptide corresponding to aa154-167, CSRPSTSSRRRAISE, of human MDM2.
MDM2 forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is overexpressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer. Applications: Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:200-1:500, Requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA, pH 8.0, is recommended. Immunohistochemistry: Frozen Immunohistochemistry: Resin Optimal working dilutions to be determined by researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Recommended Positive Control: Breast Carcinoma Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Recommended Secondary Antibodies: I1904-14C: IgG, Fc (HRP) Pab Gt x Mo I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
