MDR2 (MRP2, Multi Drug Resistance Associated Protein, cMOAT, Canalicular Multi Organic Anion Transporter), IgG2b, Clone: [6D566 M2III-5], Mouse, Monoclonal
Artikelnummer:
USB-M2825-17F
Hersteller Artikelnummer:
M2825-17F
Alternativnummer:
USB-M2825-17F-125
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
Recombinant protein corresponding to aa1339-1541 at the C-terminal of rat MDR2.
Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family. The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs. MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate. MRP2 is predominately expressed on the apical membranes in the liver and kidney proximal tubules. It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes. Applications: Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations. Immunohistochemistry (Frozen): 1:20 dilution on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse and a monoclonal mouse APAAP complex. Immunohistochemistry (Paraffin): Pretreatment with 0.01M citric acid, pH 6.0 in ddH2O at 100C for 5 min 3 times. After washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP). Note: Mouse samples are NOT suitable for use in IHC. Western Blot: 1:20-1:50. Use an IgG (HRP) anti-mouse as secondary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
