| Control peptide for M2868-45A (affinity purified antibody) and M2868-45B (antiserum). Melanocortins are regulatory peptides formed by post-translational processing of pro-opiomelanocortin. Melanocortin peptides have been suggested to perform a variety of physiological roles: control of behavior, memory, neurotrophic properties, antipyretic and modulation of immune system, etc. Their binding sites have been found distributed in many tissues (i.e. lachrymal and submandibular glands, pancreas, adipose tissue, bladder, duodenum, spleen, brain, gonadal tissues and malignant melanoma tumors). Five melanocortin receptors (MC-R) have been characterized to date. These include melanocyte- stimulating hormone receptor (MSH or MC1-R), corticoaderenal-specific ACTH receptor (MC2-R), melacortin-3 (MC3-R), melanocortin-4 (MC4-R) and melanocortin-5 receptor (MC5-R). MC3-R and MC4-R are distributed in brain. MC5-R has a broad distribution. Melanocyte Stimulating Hormone Receptor (MSH-R), also known as Melanotropin Receptor or Melanocortin-1 Receptor (MC1-R), is a 315aa transmembrane protein belonging the the family of G-Protein coupled receptors. It is a receptor for MSH (a,b,g) and ACTH. Its activity is mediated by G-Proteins, which activate adenylate cyclase. It is found in melanocytes and corticoderenal tissue, adrenal gland, leukocytes, lung, lymph node, ovary, testis, pituitary, placenta, spleen, uterus, etc. MC2-R, also know as Aderenocorticotropic Hormone Receptor (ACTH-R), is a 297aa transmembrane protein found in melanocytes and the corticoadrenal tissue. It mediated the corticotrophic effect of ACTH. MC3-R is expressed in brain. In humans, it is a 360aa protein. In miceand rats, it is 323aa. MC4-R is a 332aa transmembrane protein. It is expressed in brain, placental and gut tissues. MC5-R is a325aa transmembrane protein expressed in the adrenals, stomach, lung and brain (very low levels). It is also expressed in the three layers of adrenal cortex, predominantly in the aldosterone-producing zona glomerulosa cells. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:1000,000 using 50-100ng control peptide/well. Antibody Blocking: 5-10ug per 1ug M2868-45A (affinity purified antibody) or per 1ul M2868-45B (antiserum). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
