MET, a high affinity receptor for hepatocyte growth factor (HGF, also known as scatter factor), is a disulfide-linked heterodimer made of 45kD a- and 145kD b-subunits (1,2). The a-subunit and the amino-terminal region of the b-subunit form the extracellular domain. The remainder of the b-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of MET with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3) and is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the MET kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the MET cytoplasmic domain provides a direct binding site forGab1 (4). Altered MET levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Thus, MET is an attractive cancer therapeutic and diagnostic target (5). Sequence (linear): SDI[pY]AEI (Biotin) Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Stable for 12 months at -20C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
95+% HPLC, Mass Spec
Formulierung:
Supplied as a white to off-white lyophilized powder.
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