MlsI (BalI)

Produktübersicht

• Artikelname: MlsI (BalI)
• Artikelnummer: USB-M4130
• Hersteller Artikelnummer: M4130
• Alternativnummer: USB-M4130-200,USB-M4130-1000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-T G G C C A-3 3-A C C G G T-5 Source: Micrococcus luteus Ng 16-122 Concentration: 5u/ul Unit Definition: One unit is defined as the amount of MlsI required to digest 1ug of lambda DNA dcm in 1 hour at 37C in 50ul of reaction buffer. Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37C). R1625-04: Restriction Enzyme Buffer E, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37C. Storage and Dilution Buffer: Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Thermal Inactivation: MlsI is inactivated by incubation at 65C for 20min. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with MlsI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with MlsI, more than 90% of the DNA fragments can be ligated at a 5-termini concentration of 0.9uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of single- stranded or double-stranded labeled oligonucleotide was observed after incubation with 10 units of MlsI for 4 hours. Stability during Prolonged Incubation: A minimum of 0.5units of MlsI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Number of Recognition Sites in DNA: Lambda: 18 PhiX174: 0 M13mp18/19: 1 pBR322: 1 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 Digestion of Agarose-embedded DNA: A minimum of 20 units of MlsI is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Recommended Protocol for Digestion: Add: Nuclease free water: 16ul R1625-04: 2ul DNA (0.5-1ug/ul): 1ul MlsI: 0.5-2ul Mix gently, spin down for a few seconds. Incubate at 37C for 1-2 hours. Protocol for Digestion of PCR products directly after amplification: Add: PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA) Nuclease free water: 18ul R1625-04: 2ul MlsI: 1-2ul Mix gently, spin down for a few seconds. Incubate at 37C for 1-16 hours. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.