Monoamine Oxidase Assay Kit, BioAssay(TM)

Produktübersicht

• Artikelname: Monoamine Oxidase Assay Kit, BioAssay(TM)
• Artikelnummer: USB-M4450-05
• Hersteller Artikelnummer: M4450-05
• Alternativnummer: USB-M4450-05-100
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Monoamine Oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression. MAO Assay Kit provides a convenient fluorometric means to measure MAO enzyme activity. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorometric method (lem/ex=585/530nm). The assay is simple, sensitive, stable and high- throughput adaptable. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L MAO activity can be quantified. Homogeneous and convenient. Mix-incubate-measure type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Applications: MAO-A/B activity determination in biological samples. Evaluation and screening for MAO inhibitors. Kit Contents: M4450-05A: Assay Buffer: 1x12ml M4450-05B: p-Tyramine: 1x300ul M4450-05C: Dye Reagent: 1x120ul M4450-05D: HRP Enzyme: 1x120ul M4450-05E: Pargyline 20mM: 1x50ul M4450-05F: Clorgyline 20mM: 1x50ul M4450-05G: Hydrogen Peroxide, 3% H2O: 1x100ul Storage and Stability: Store all components at -20C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.