MunI (MfeI)

Produktübersicht

• Artikelname: MunI (MfeI)
• Artikelnummer: USB-M9219
• Hersteller Artikelnummer: M9219
• Alternativnummer: USB-M9219-300,USB-M9219-1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-C A A T T G-3 3-G T T A A C-5 Concentration: ~10u/ul Source: E. coli that carries the cloned munIR gene from Mycoplasma unidentified. Storage Buffer: Enzyme is supplied in 10mM Tris-HCl pH7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Diluent Buffer: 10mM Tris-HCl pH7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with MunI. Ligation/Recutting Assay: The litigation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phospatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of MunI for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Stability during Prolonged Incubation: A minimum of 0.1 units of MunI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: MunI is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of MunI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: XapI, EcoRI, TasI Number of Recognition Sites in DNA: Lambda: 8 PhiX174: 1 M13mp18/19: 0 pBR322: 0 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 Methylation Effect on Digestion: Dam, Dcm, CpG, EcoKI: Never overlaps - no effect. EcoBI: May overlap - effect undetermined. Unit Definition: One unit is defined as the amount of MunI required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer. Supplied with: R1625 - Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-02 - Restriction Enzyme Buffer C, 10X: Dilute to 1X for 100% MunI digestion. 1X buffer compostion is 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM sodium chloride and 0.1mg/ml BSA. Incubate at 37C Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap